CELL LINES
Contributor Information
- Name Anne Lykkesfeldt
- Institute Danish Cancer Society, Denmark
Tool Details
- Tool name: MCF7/TAMR-7 Cell line
- Alternate names: MCF-7/TAMR-7; TAMR-7
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF7 S0.5
- Organism: Human
- Tissue: Breast
- Gender: Female
- Cancer type: Breast cancer
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Application: Investigate signalling pathways involved in tamoxifen resistance
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Description: MCF7/TAMR-7 cell line is a stable tamoxifen-resistant subline. The cell line has been established in tissue culture after long term treatment with 1uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat.
This cell line was produced as an adaptation of an original cell line (MCF7/S0.5), as a model cell system to study the effects of tamoxifen resistant cancer growth. MCF7/TAMR-7 cells are oestrogen receptor positive and progesterone receptor negative.
Previous applications of this cell line include treatment with steroidal antiestrogens. MCF7/TAMR-7 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance. - Research area: Cancer; Drug development
- Production details: The parental cell line for the MCF7/TAMR-7 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-7 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
- Cellosaurus ID: 1D43
- For Research Use Only
Target Details
- Target: Oestrogen receptor
Application Details
- Application: Investigate signalling pathways involved in tamoxifen resistance
- Application notes: MCF7/TAMR-7 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-7 cells are growth inhibited by the pure antioestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-7 cell. The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and after 28 days of tamoxifen treatment, tamoxifen was omitted from the medium for 22 days. After 19 passages without tamoxifen (passage 372) the cells underwent a second treatment with tamoxifen which initially reduced cell growth rate, but around 390-400 the growth rate of the tamoxifen resistant cell lines was close to the growth rate of the parental MCF7/S0.5 cells.
Handling
- Format: Frozen
- Growth medium: Phenol red-free DMEM:Ham's F-12 containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.
- • Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577.
- • Pedersen et al. 2014. Int J Oncol. 45(5):2167-2175. PMID: 25175082.
- • Lundqvist et al. 2014. Steroids. 85:30-35. PMID: 24747771.
- • Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470.
- • Larsen et al. 2012. Int J Oncol. 41(5):1863-1870. PMID: 22961366.
- • Frankel et al. 2007. Breast Cancer Res Treat. 104(2):165-179. PMID: 17061041.
- • Berstein et al. 2003. Endocr Relat Cancer. 10(2):267-277. PMID: 12790788.
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