ANTIBODIES

Contributor Information
- Name Laurie Milner
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Huppert et al.2000. Nature. 405[6789):966-70. PMID: 10879540
Tool Details
- Tool name: Anti-mouse Notch1
- Clone: Mn1a
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human, Mouse.
- Host: Mouse
- Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
- Description: Antibody mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.
- Isotype: IgG1
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: Notch1 protein
- Target background: Intracellular domain of Notch1 protein, mainly its activated form. The Notch genes encode single-pass transmembrane receptors that transduce the extracellular signals responsible for cell fate determination during several steps of metazoan development.
Application Details
- Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage conditions: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Related Tools
References
- • Huppert et al.2000. Nature. 405[6789):966-70.PMID: 10879540