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Contributor Information

  • Name Daniel Geraghty
  • Institute Fred Hutchinson Cancer Research Center
  • Primary citation lshitani et al.2003. Journal of lmmunology. 171(3):1376-84. PMID: 12874228

Tool Details

  • Tool name: Anti-HLA-G [o1G]
  • Clone: 01G
  • Tool type: Antibody
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Skin cancer; Breast cancer; Genitourinary cancer; Blood cancer
  • Molecular weight of the target: The heavy chain is approximately 45 kDa
  • Application: Immunohistochemistry (Frozen), Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation. No western
  • Description: HLA-G has long been assumed a major factor in immune accommodation because of its localized expression and low levels of allelic polymorphism. This MoAb were developed to examine the role of all three nonclassical class I Ags in the maternal immune response during pregnancy. 0lG was developed to react specifically with the membrane form of HLA-G.
  • Isotype: IgG1
  • Research area: Immunology; Developmental biology

  • For Research Use Only

Target Details

  • Target: Membrane-bound form of HLA-G (full-length HLA-G1)
  • Target molecular weight: The heavy chain is approximately 45 kDa
  • Target background: HLA-G are expressed in the placenta, a membrane form and a soluble form, the latter containing an altered carboxyl terminu. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (?2 microglobulin). The heavy chain is anchored in the membrane.

Application Details

  • Application: Immunohistochemistry (Frozen), Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation. No western
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)

Handling

  • Storage buffer: PBS, pH 7.4
  • Storage conditions: 4° C, do not freeze

Documentation

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References

  • •  lshitani et al.2003.Journal of lmmunology. 171(3):1376-84.PMID: 12874228
  • •  Lee et al.1995. Immunity.(5):591-600.PMID: 7584149