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Contributor Information

  • Name Stephen West
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-RuvC [RuvC 5G9/3] mAb
  • Clone: RuvC 5G9/3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: E.coli
  • Host: Mouse
  • Molecular weight of the target: 79 kDa
  • Application: ELISA ; RIA ; WB
  • Strain: Balb/c
  • Description: RuvC (an endonuclease) cleaves Holliday junctions.
  • Immunogen: Ruv C protein from E. Coli.
  • Isotype: IgG2b
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: RuvC
  • Target molecular weight: 79 kDa
  • Target background: RuvC (an endonuclease) cleaves Holliday junctions.

Application Details

  • Application: ELISA ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

Documentation

References

  •   Davies et al. 1998. Curr Biol. 8(12):725-7. PMID: 9637927.
  •   Formation of RuvABC-Holliday junction complexes in vitro.
  •   Eggleston et al. 1997. Cell. 89(4):607-17. PMID: 9160752.
  •   In vitro reconstitution of the late steps of genetic recombination in E. coli.