ANTIBODIES

Contributor Information
- Name Stephen West
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: Anti-RuvC [RuvC 5G9/3] mAb
- Clone: RuvC 5G9/3
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: E.coli
- Host: Mouse
- Molecular weight of the target: 79 kDa
- Application: ELISA ; RIA ; WB
- Strain: Balb/c
- Description: RuvC (an endonuclease) cleaves Holliday junctions.
- Immunogen: Ruv C protein from E. Coli.
- Isotype: IgG2b
- Research area: Genetics
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: RuvC
- Target molecular weight: 79 kDa
- Target background: RuvC (an endonuclease) cleaves Holliday junctions.
Application Details
- Application: ELISA ; RIA ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- • Davies et al. 1998. Curr Biol. 8(12):725-7. PMID: 9637927.
- • Formation of RuvABC-Holliday junction complexes in vitro.
- • Eggleston et al. 1997. Cell. 89(4):607-17. PMID: 9160752.
- • In vitro reconstitution of the late steps of genetic recombination in E. coli.