ANTIBODIES
Contributor Information
- Name Elizabeth A. Wayner, William Carter
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Wayner et al. 1988. Journal of Cell Biology.107(5):1881-91. PMID: 2846588
Tool Details
- Tool name: Anti-integrin a5
- Clone: P1D6
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 115 kDa
- Application: FFPE, Function Blocking, Immunoprecipitation, Western Blot
- Description: MoAb P1D6 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?5/?1. P1D6 neutralizes binding of the ?5/?1 receptor to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. P1D6 recognizes native protein only.
- Immunogen: non-trypsinized WI-38 VA13 or HT-1080 cells
- Isotype: IgG1
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: Integrin alpha-5 (CD49e)
- Target molecular weight: 115 kDa
- Target background: ?5?1 complex of Collagen receptors: I, II, III, IV.
Application Details
- Application: FFPE, Function Blocking, Immunoprecipitation, Western Blot
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage conditions: . For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Wayner et al. 1988. Journal of Cell Biology.107(5):1881-91.PMID: 2846588
- • Mould et al.1997.J Biol CHem.272(28):17283-92.PMID: 9211865
- • Wayner.2007. Methods Enzymol.426:117-53.PMID: 17697883
- • Wayner et al.1989. Journal of Cell Biology. 109(3):1321-30
- • Carter et al.1990. J Cell Biol.111(6 Pt 2):3141-54.PMID: 2269668
- • Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668
- • Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064
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