ANTIBODIES
Contributor Information
- Name Elizabeth A. Wayner, William Carter
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Wayner et al. 1989. Journal of Cell Biology. 109(3):1321-30. PMID: 2527858
Tool Details
- Tool name: Anti-integrin a4 [P3E3]
- Clone: P3E3
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 70-150kDa
- Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
- Description: MoAb P3E3 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?4/?1. P3E3 recognized epitopes on the alpha 4 subunit and completely inhibited the adhesion of peripheral blood and cultured T lymphocytes to a 38-kD tryptic fragment of plasma fibronectin containing the carboxy-terminal Heparin II domain and part of the type III connecting segment. The ligand in type III connecting segment (IIICS ) for ?4/?1 was the CS-1 region previously defined as an adhesion site for melanoma cells.
- Immunogen: HT1080 human fibrosarcoma cell line
- Isotype: IgG2b
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: Integrin alpha-4 (CD49d)
- Target molecular weight: 70-150kDa
- Target background: A fibronectin receptor that is identical to the integrin receptor ?4?1. CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit ?
Application Details
- Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Wayner et al. 1989. Journal of Cell Biology. 109(3):1321-30. PMID: 2527858
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