Product Image

Contributor Information

  • Name David Lane
  • Institute University of Dundee
  • Primary citation Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.

Tool Details

  • Tool name: Anti-p53 [Pab DO-2]
  • Clone: Pab DO-2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 53 kDa
  • Application: IHC ; IP ; WB
  • Description: A monoclonal p53 antibody, the epitope recognised is in the N terminus of p53.
  • Immunogen: p53
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Genetics

  • For Research Use Only

Target Details

  • Target: p53 (N terminus)
  • Target molecular weight: 53 kDa
  • Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.

    Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy.

    DO-2 recognises an epitope specific for the N terminus region of p53, between aa 10-16.

Application Details

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-TagYFP (Rabbit) "Brainbow"

#155275
Product Image

Anti-S100A9 [6G4]

#151881
Product Image

Anti-HPV16E2 [TVG 261]

#151167

References

  • •  Sonnemann et al. 2011. Eur J Cancer. 47(9):1432-41. PMID: 21334198.
  • •  Zaman et al. 2007. Cancer Res. 67(20):10078-86. PMID: 17942942.
  • •  Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.
  • •  Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.