ANTIBODIES
Contributor Information
- Name Elizabeth A. Wayner, William Carter
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727
Tool Details
- Tool name: Anti-integrin a3 not inhibiting
- Clone: P1F2
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 80-90kDa
- Application: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
- Description: Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin ?3 (P1F2) is a mouse monoclonal antibody developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. P1F2 stimulated attachment of cell adhesion/collagen binding or had no effect, and bound to types I and VI collagen receptors but not to fibronectin.
- Immunogen: HT1080 human fibrosarcoma cell line
- Isotype: IgG1
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: Integrin alpha-3 (CD49b)
- Target molecular weight: 80-90kDa
- Target background: ?2?1 complex of Collagen receptors: I, II, III, IV.
Application Details
- Application: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
- • Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668
- • Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064
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