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Contributor Information

  • Name Elizabeth A. Wayner William Carter,
  • Institute Fred Hutchinson Cancer Research Center
  • Primary citation Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727

Tool Details

  • Tool name: Anti-CD44 [P1H5]
  • Clone: P1H5
  • Tool type: Antibody
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 125-145 kDa
  • Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
  • Description: MoAb is a mouse monoclonal antibody raised against HT-1080. MoAb was developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. This antibody inhibited cell adhesion to type VI and I collagens only and not to fibronectin or laminin.
  • Immunogen: HT1080 human fibrosarcoma cell line
  • Isotype: IgG1
  • Research area: Cell biology

  • For Research Use Only

Target Details

  • Target: CD44
  • Target molecular weight: 125-145 kDa
  • Target background: CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Antibody bound and inhibited cell adhesion to type VI and I collagens (and bound type III, IV, and V collagens with no inhibition) only and not to fibronectin or laminin

Application Details

  • Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

Documentation

References

  •   Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
  •   Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668
  •   Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064