Product Image

Contributor Information

  • Name Thomas Spies; Veronika Groh
  • Institute Fred Hutchinson Cancer Research Center
  • Primary citation BauerS et al. 1999. Science. 285(5428):727-9. PMID: 10426993

Tool Details

  • Tool name: Anti-NKG2D monoclonal antibody [5c6]
  • Clone: 5C6
  • Tool type: Antibody
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Reactivity: Human, Mouse
  • Host: Mouse
  • Cancer type: Lung cancer; Breast cancer; Genitourinary cancer; Gynaecologic cancer; Digestive / Gastrointestinal cancer
  • Molecular weight of the target: 42kDa
  • Application: IP, Function Blocking
  • Strain: Can be used for: blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation.
  • Description: Anti-human NKG2D is a monoclonal antibody to NKD2D. NKG2D is a MICA Receptor found on ?? T cells and CD8+ ?? T cells, natural killer (NK) cells and is frequently expressed in epithelial tumors. The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation
  • Isotype: IgG1, ?
  • Research area: Immunology

  • For Research Use Only

Target Details

  • Target: MICA receptor NKG2D, Collagen type VI
  • Target molecular weight: 42kDa
  • Target background: NKG2D is a receptor for MICA detected on most ?? T cells, CD81 ?? T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of ?? T cells and NK cells against transfectants and epithelial tumor cells expressing MICA.

Application Details

  • Application: IP, Function Blocking
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

Documentation

Related Tools

Product Image

Anti-SHIP

#161664
Product Image

Anti-Myeloperoxidase (MPO) scFv (B10B) [B10BscFv]

#156520
Product Image

Anti-Sucrase-Isomaltase [mglu3]

#151697

References

  • •  BauerS et al. 1999. Science. 285(5428):727-9. DOI: 10.1126/science.285.5428.727
  • •  V. Groh et al.1998. Science 279(1737).PMID: 9497295