ANTIBODIES
Contributor Information
- Institute The University of Iowa
Tool Details
- Tool name: Anti-1,4-dihydropyridine receptor [IIF7]
- Alternate names: DHPR
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Rabbit
- Host: Mouse
- Molecular weight of the target: 170
- Application: IP
- Description: 1,4-dihydropyridine receptor is present in skeletal muscle and associated with voltage-dependent calcium channels. This antibody was developed for immunoprecipitating radiolabelled dyhydropyridine receptors from digitonin solubilized triads. This antibody and it's related antibodies recognise the 170 kDa protein on nitrocellulose transfers of skeletal muscle triads, transverse tubular membreanes, and purified receptor.
- Immunogen: 0.5 mg rabbit skeletal muscle triads emulsified in Freund's complete adjuvant. After 4 weeks, immunization with 0.5 mg rabbit skeletal muscle triad in Freund's incomplete adjuvant was repeated 3-4 additional times at two week intervals. Two IP injections of GlcNAc-eluted dihydropyridine receptor were giving followed by 40 mg IV purified dihydropyridine receptor two days prior to fusion.
- Research area: Tissue-specific biology
- Myeloma used: NS1
- For Research Use Only
Target Details
- Target: 1,4-dihydropyridine receptor
- Target molecular weight: 170
- Target background: 1,4-dihydropyridine receptor is present in skeletal muscle and associated with voltage-dependent calcium channels. This antibody was developed for immunoprecipitating radiolabelled dyhydropyridine receptors from digitonin solubilized triads. This antibody and it's related antibodies recognise the 170 kDa protein on nitrocellulose transfers of skeletal muscle triads, transverse tubular membreanes, and purified receptor.
Application Details
- Application: IP
Handling
- Format: Liquid
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Leung et al. 1987. J Biol Chem. 262(17):7943-6. PMID: 2439496.
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