ANTIBODIES
Contributor Information
- Name Demin Li ;Alison Banham
- Institute University of Oxford
Tool Details
- Tool name: Anti-T-cell receptor mimic against WT p53 [T1-116C]
- Alternate names: Anti-p53 TCRm monoclonal antibody
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Host: Mouse
- Immunogen: HLA-A*0201/p53 tetramers. To generate the tetramers, HLA-A_0201 (15 mg), b2m (12.5 mg), and peptide (5 mg) were added into 500 mL of refolding buffer and refolded for 48 hours. The refolding complex was concentrated and buffer exchanged to 10 mmol/L Tris-HCl, pH8.0, and then biotinylated with BirA protein biotin ligase. Biotinylated protein was then separated by FPLC, and HLA-A2/b2m/peptide monomers were isolated and subsequently stored at -80C. Aliquots were thawed and tetramerized with Extravidin on use.
- Isotype: IgG1
- Research area: Immunology; Immuno-oncology
- Myeloma used: NS0
- For Research Use Only
Target Details
- Target: WT p53, Wild Type p53
Application Details
Handling
- Format: Liquid
- Shipping conditions: Shipping at 4°C
Documentation
Related Tools
References
- • GB2016052674
- • Li et al. 2017. Cancer Res. 77(10):2699-2711. PMID: 28363997.
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