Product Image

Contributor Information

  • Institute Yale University

Tool Details

  • Tool name: Anti-CD3 [500.A2]
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Hamster
  • Cancer type: Head and neck cancer
  • Description: Monoclonal antibody capable of activating T cells and cloned T cell lines. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3? and 2X CD3?), associates with the T cell antigen receptor (TCR. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. Binding of anti-CD3 (500.A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
  • Immunogen: 25-kDa e chain of the T-cell receptor-associated CD3 complex
  • Immunogen UniProt ID: A0A3Q0CNZ4
  • Isotype: IgG
  • Research area: Immunology

  • For Research Use Only

Target Details

  • Target: CD3
  • Target background: Monoclonal antibody capable of activating T cells and cloned T cell lines. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3d and 2X CD3), associates with the T cell antigen receptor (TCR. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. Binding of anti-CD3 (500.A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Application Details

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-Bordotella avium 41-kDa Surface Protein [PF8]

#157691
Product Image

Anti-Fast Skeletal Muscle Troponin T [F24]

#151598
Product Image

Anti-Follicular Dendritic Cell Antigen [BU10]

#151496

References

  • •  Chen et al. 2019. JCI Insight. 4(22):. PMID: 31723059.
  • •  Trittel et al. 2019. Sci Rep. 9(1):16362. PMID: 31704965.
  • •  Czarnewski et al. 2019. Nat Commun. 10(1):2892. PMID: 31253778.
  • •  Zwick et al. 2019. Front Immunol. 10:222. PMID: 30809231.
  • •  Shahbaz et al. 2018. PLoS Biol. 16(12):e2006649. PMID: 30550561.
  • •  Maj et al. 2017. Nat Immunol. 18(12):1332-1341. PMID: 29083399.
  • •  Long et al. 2016. Genes (Basel). 8(1):. PMID: 28035981.
  • •  Portoles et al. 1989. J Immunol. 142(12):4169-75. PMID: 2470817.