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Contributor Information

  • Institute Netherlands Cancer Institute

Tool Details

  • Tool name: Anti-CD27 [LG8A6]
  • Alternate names: Tumor Necrosis Factor Receptor Superfamily, Member 7; TNFRSF7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Hamster
  • Molecular weight of the target: 60 kDa
  • Application: ELISA ; FACS ; IHC ; IP
  • Strain: Armenian
  • Description: CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-Î?B and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signalling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
  • Immunogen: Armenian hamster fibroblasts transfected with the mouse CD27 DNA.
  • Isotype: IgG
  • Research area: Apoptosis and autophagy; Cell signaling and signal transduction; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: CD27
  • Target molecular weight: 60 kDa
  • Target background: CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-B and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signalling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Application Details

  • Application: ELISA ; FACS ; IHC ; IP

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Hendriks et al. 2000. Nat Immunol. 1(5):433-40. PMID: 11062504.
  •   Gravestein et al. 1996. J Exp Med. 184(2):675-85. PMID: 8760821.
  •   Gravestein et al. 1995. Int Immunol. 7(4):551-7. PMID: 7547681.