ANTIBODIES

Contributor Information
- Institute Netherlands Cancer Institute
Tool Details
- Tool name: Anti-CD27 [LG8A6]
- Alternate names: Tumor Necrosis Factor Receptor Superfamily, Member 7; TNFRSF7
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human ; Mouse
- Host: Hamster
- Molecular weight of the target: 60 kDa
- Application: ELISA ; FACS ; IHC ; IP
- Strain: Armenian
- Description: CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-ÄÂ?B and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signalling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
- Immunogen: Armenian hamster fibroblasts transfected with the mouse CD27 DNA.
- Isotype: IgG
- Research area: Apoptosis and autophagy; Cell signaling and signal transduction; Immunology
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: CD27
- Target molecular weight: 60 kDa
- Target background: CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-B and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signalling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Application Details
- Application: ELISA ; FACS ; IHC ; IP
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Hendriks et al. 2000. Nat Immunol. 1(5):433-40. PMID: 11062504.
- • Gravestein et al. 1996. J Exp Med. 184(2):675-85. PMID: 8760821.
- • Gravestein et al. 1995. Int Immunol. 7(4):551-7. PMID: 7547681.