ANTIBODIES
Contributor Information
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti-SUN1 [12.10F]
- Alternate names: Protein unc-84 homolog A antibody, Sad1 and UNC84 domain containing 1 antibody, Sad1/unc 84 protein like 1 antibody, Sad1/unc-84 protein-like 1 antibody, Sun domain-containing protein 1 antibody, Sun1 antibody, SUN1_HUMAN antibody
- Clone: 12.10F
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mouse ; Rat
- Host: Mouse
- Molecular weight of the target: 102 kDa
- Application: IF ; WB
- Strain: C57BL/6
- Description: A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility. Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent.
- Immunogen: GST fused to residues encoded by exons 6, 8, 10
- Immunogen UniProt ID: Q9D666
- Isotype: IgG2a kappa
- Research area: Cell signaling and signal transduction; Developmental biology
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: SUN-1
- Target molecular weight: 102 kDa
- Target background: A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility. Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent.
Application Details
- Application: IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
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