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Contributor Information

  • Name Ciaran Morrison
  • Institute National University of Ireland, Galway; Centre For Chromosome Biology

Tool Details

  • Tool name: Anti-CEP164 [1F3G10]
  • Alternate names: Centrosomal protein of 164 kDa, KIAA152, NPHP15
  • Clone: [1F3G10]
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 164 kDa (runs c.190)
  • Application: IHC ; IF ; IP ; WB
  • Description: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.
  • Immunogen: Recombinant human CEP164 amino acids 6-296 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology; Metabolism
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Anti-CEP164 [1F3G10]
  • Target molecular weight: 164 kDa (runs c.190)
  • Target background: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Flynn et al. 2020. Hum Mol Genet. 29(3):407-417. PMID: 31868218.
  • •  Khouj et al. 2019. J Cell Sci. 132(19):. PMID: 31492759.
  • •  Ogungbenro et al. 2018. J Cell Biol. 217(4):1205-1215. PMID: 29440264.
  • •  Sieben et al. 2018. Nat Methods. 15(10):777-780. PMID: 30275574.
  • •  Daly et al. 2016. J Cell Sci. 129(9):1769-74. PMID: 26966185.