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Contributor Information

  • Institute BioServ UK Ltd

Tool Details

  • Tool name: Anti-Protein Kinase C a [133]
  • Alternate names: Protein kinase C alpha, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
  • Clone: 133
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mammals
  • Host: Mouse
  • Molecular weight of the target: 75 kDa
  • Application: IHC ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody which specifically detects the ? isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKC?) is involved in the regulation of cell proliferation during cell cycle progression. PKC-? is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the ? isoform of PKC and binds to a sequence at the C terminus of PKC? to detect its expression in vitro.
  • Immunogen: Recognizes sequence PQFVHPILQSAV at the C terminus at PKCa
  • Immunogen UniProt ID: P17252
  • Isotype: IgG2a
  • Research area: Cancer; Neurobiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Protein Kinase C a
  • Target molecular weight: 75 kDa
  • Target background: Monoclonal antibody which specifically detects the a isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKCa) is involved in the regulation of cell proliferation during cell cycle progression. PKC-a is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the a isoform of PKC and binds to a sequence at the C terminus of PKCa to detect its expression in vitro.

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.
  • •  Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.
  • •  Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.
  • •  Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.
  • •  Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.
  • •  Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.
  • •  Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Ca consensus site.
  • •  Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.
  • •  Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
  • •  Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.
  • •  High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.
  • •  Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.
  • •  Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.