ANTIBODIES

Contributor Information
- Name Vincenzo D'Angiolella
- Institute University of Oxford
Tool Details
- Tool name: Anti-phospho-Sufu
- Alternate names: Suppressor of fused homolog, SUFUH
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Polyclonal
- Conjugate: Unconjugated
- Reactivity: Human ; Mouse
- Host: Rabbit
- Molecular weight of the target: ~65 kDa
- Application: IF ; WB
- Description: Anti-phospho-Sufu polyclonal antibody recognises wild type sufu which is phosphorylated at serine 352. The antibody specificity for the phosphorylated version has been confirmed; it does not react against a non-phosphorylated version of Sufu which has serine 352 substituted by alanine. Sufu is a negative regulator in the hedgehog signaling pathway which directs cell proliferation and patterning during embryogenesis. Sufu down-regulates GLI1- and GLI2-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. Required for normal embryonic development. Sufu is ubiquitous in adult tissues. Heterozygous loss of Sufu, in conjunction with the loss of p53, leads to the development of medulloblastoma and rhabdomyosarcoma. Somatic Sufu mutations have been identified in multiple other malignancies, including prostate cancer.
- Immunogen: Sufu peptide: Hu #347~360: CLESDS-pS-pT-AIIPHEL
- Research area: Cancer; Developmental biology; Genetics
- For Research Use Only
Target Details
- Target: Sufu (UniProt ID: Q9UMX1)
- Target molecular weight: ~65 kDa
- Target background: Anti-phospho-Sufu polyclonal antibody recognises wild type sufu which is phosphorylated at serine 352. The antibody specificity for the phosphorylated version has been confirmed; it does not react against a non-phosphorylated version of Sufu which has serine 352 substituted by alanine. Sufu is a negative regulator in the hedgehog signaling pathway which directs cell proliferation and patterning during embryogenesis. Sufu down-regulates GLI1- and GLI2-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. Required for normal embryonic development. Sufu is ubiquitous in adult tissues. Heterozygous loss of Sufu, in conjunction with the loss of p53, leads to the development of medulloblastoma and rhabdomyosarcoma. Somatic Sufu mutations have been identified in multiple other malignancies, including prostate cancer.
Application Details
- Application: IF ; WB
Handling
- Format: Liquid
- Concentration: 2 mg/ml
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Raducu et al. 2016. EMBO J. 35(13):1400-16. PMID: 27234298.
- • SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development.