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Contributor Information

  • Name David Lane
  • Institute University of Dundee

Tool Details

  • Tool name: Anti-p53 [DO-12]
  • Alternate names: p53
  • Clone: DO-12
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 53 kDa
  • Application: IF ; WB
  • Description: Anti-p53 DO-12 antibody recognises the human cellular tumour antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein upregulated in response to DNA damage and is found in a wide variety of transformed cells. DO-12 is directed to an epitope in the DNA binding region of p53, between amino acids 250-270. It is believed that twelve isoforms of human p53 are produced by alternative splicing and promotor usage; the epitope recognized by clone DO-12 is reportedly present in all isoforms. DO-12 recognises mutant and unfolded p53.
  • Immunogen: Recombinant human p53
  • Immunogen UniProt ID: P04637
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Immunology

  • For Research Use Only

Target Details

  • Target: Human TP53 / p53
  • Target molecular weight: 53 kDa
  • Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.

    Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy.

    Twelve isoforms of p53 have been described: p53α, p53β, p53γ, Δ40p53α, Δ40p53β, Δ40p53γ, Δ133p53α, Δ133p53β, Δ133p53γ, Δ160p53α, Δ160p53β, and Δ160p53γ. Depending on the location of the epitope, antibodies may detect different isoforms of p53. Mutations to p53 can cause conformational changes that expose different epitopes. DO-12 binds to an epitope between amino acids 250 and 270, which is located between conserved domain IV and V on an exposed turn of the protein structure. The epitope recognised by clone DO-12 is believed to be present in all isoforms. DO-12 recognises the mutant conformation of p53.

Application Details

  • Application: IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Pang et al. 2013. Vet J. 196(3):414-23. PMID: 23219486.
  •   Fujita et al. 2009. Nat Cell Biol. 11(9):1135-42. PMID: 19701195.
  •   Bourdon et al. 2005. Genes Dev. 19(18):2122-37. PMID: 16131611.
  •   Vojtesek et al. 1995. Oncogene. 10(2):389-93. PMID: 7530828.