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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Plakoglobin [VB3]
  • Alternate names: Gamma Catenin
  • Clone: VB3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Rabbit
  • Application: IP ; IF ; IP ; WB
  • Description: Plakoglobin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Immunogen: Peptide - NH2-CIDTYSDGLRPPYPTADH-COOH
  • Research area: Cell biology

  • For Research Use Only

Target Details

  • Target: Plakoglobin
  • Target background: Plakoglobin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

Application Details

  • Application: IP ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: Serum
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Erasmus et al. 2016. Nat Commun. 7:13542. PMID: 27922008.
  • •  Defining Fn interactions during biogenesis of epithelial junctions.
  • •  Ryan et al. 2012. J Cell Sci. 125(Pt 13):3202-9. PMID: 22454524.
  • •  Plakoglobin-dependent regulation of keratinocyte apoptosis by Rnd3.
  • •  Zhu et al. 1999. Development. 126(10):2285-98. PMID: 10207152.
  • •  beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion.
  • •  Braga et al. 1995. Cell Adhes Commun. 3(3):201-15. PMID: 8846022.
  • •  Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
  • •  Näthke et al. 1994. J Cell Biol. 125(6):1341-52. PMID: 8207062.
  • •  Defining interactions and distributions of cadherin and catenin complexes in polarized epithelial cells.