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Contributor Information

  • Institute A*STAR Accelerate Technologies Pte Ltd

Tool Details

  • Tool name: Anti-Bam32 [4H9]
  • Clone: 4H9
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: WB
  • Description: B lymphocyte-associated adaptor protein (Bam32) is 32 kDa and is expressed in high levels in the germinal center (GC) of B cells. It consists of a src homology 2 (SH2) domain at the NH2 terminal which binds phospholipase C (PLC) gamma 2 and a pleckstrin homology (PH) domain at the COOH-terminal. Bam32 acts as an adaptor that integrates protein tyrosine kinase (PTK) and phosphatidylinositol 3-kinase (PI3K) necessary for signaling in B cells. Bam32 is recruited to the plasma membrane upon B cell receptor (BCR) ligation. BCR ligation induces colocalization of Bam32 with lipid rafts, clathrin, and actin filaments. BCR internalization which is actin dependent occurs subsequently. Bam32 also play a part in B cell proliferation.
  • Immunogen: GST-Bam32 fusion protein
  • Isotype: IgG1
  • Research area: Cell signaling and signal transduction; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: B lymphocyte-associated adaptor protein
  • Target background: B lymphocyte-associated adaptor protein (Bam32) is 32 kDa and is expressed in high levels in the germinal center (GC) of B cells. It consists of a src homology 2 (SH2) domain at the NH2 terminal which binds phospholipase C (PLC) gamma 2 and a pleckstrin homology (PH) domain at the COOH-terminal. Bam32 acts as an adaptor that integrates protein tyrosine kinase (PTK) and phosphatidylinositol 3-kinase (PI3K) necessary for signaling in B cells. Bam32 is recruited to the plasma membrane upon B cell receptor (BCR) ligation. BCR ligation induces colocalization of Bam32 with lipid rafts, clathrin, and actin filaments. BCR internalization which is actin dependent occurs subsequently. Bam32 also play a part in B cell proliferation.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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