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Contributor Information

  • Institute University of Georgia

Tool Details

  • Tool name: Anti-Actin8 [mAbGPa]
  • Alternate names: ACT8
  • Clone: mAbGPa
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Arabidopsis
  • Host: Mouse
  • Molecular weight of the target: 45 kDa
  • Application: ELISA ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins. This mouse monoclonal antibody was generated against plant actin ACT8 and recognizes all tested subclasses of plant actins (arabidopsis ACT1, 2, 3, 4, 7, 8, 11, 12) and Dictyosetelium actin.
  • Immunogen: Purified Arabidopsis actin (ACT8)
  • Isotype: IgG2b
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: ArabidopsisActin (ACT8)
  • Target molecular weight: 45 kDa
  • Target background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins. This mouse monoclonal antibody was generated against plant actin ACT8 and recognizes all tested subclasses of plant actins (arabidopsis ACT1, 2, 3, 4, 7, 8, 11, 12) and Dictyosetelium actin.

Application Details

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Kandasamy et al. 2012. Plant Cell. 24(5):2041-57. PMID: 22589468.
  • •  Plant vegetative and animal cytoplasmic actins share Fn competence for spatial development with protists.
  • •  Kandasamy et al. 2001. Plant Cell. 13(7):1541-54. PMID: 11449050.
  • •  One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation.
  • •  Kandasamy et al. 1999. Plant J. 18(6):681-91. PMID: 10417720.
  • •  The late pollen-specific actins in angiosperms.