ANTIBODIES
Contributor Information
- Name Rupert Fray
- Institute Absolute Antibody ; University of Nottingham
Tool Details
- Tool name: Anti-N6-methyladenosine (m6A) [17-3-4-1] rAb
- Clone: 17-3-4-1
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Recombinant
- Conjugate: Unconjugated
- Reactivity: Human ; Mouse ; Saccharomyces cerevisiae
- Host: Mouse
- Application: IP ; DB
- Description: Recombinant monoclonal antibody which binds to m6A modification found in RNA and allows for analysis of methylated transcriptomes. This can be used to investigate how m6A modifications regulates gene expression. Background and Research Application N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalysed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. The m6A antibody was created to identify m6A containing messages within RNA and helped identify the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself as being methylated in RNA transcripts. This is a recombinant version of the anti-N6-methyladenosine monoclonal antibody. Anti-m6A. helped narrow down the position of m6A in IME2 as towards the 3ĂÂ end. Anti-N6-methyladenosine will allow for analysis of methylated transcriptomes, and could aid understanding in how the m6A base modification regulates gene expression.
- Immunogen: Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine
- Immunogen UniProt ID: Q8BGW1
- Isotype: IgG1 kappa
- Research area: Genetics
- For Research Use Only
Target Details
- Target: N6-methyladenosine-5'-mono-phosphate
- Target background: Recombinant monoclonal antibody which binds to m6A modification found in RNA and allows for analysis of methylated transcriptomes. This can be used to investigate how m6A modifications regulates gene expression. Background and Research Application N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalysed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. The m6A antibody was created to identify m6A containing messages within RNA and helped identify the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself as being methylated in RNA transcripts. This is a recombinant version of the anti-N6-methyladenosine monoclonal antibody. Anti-m6A. helped narrow down the position of m6A in IME2 as towards the 3' end. Anti-N6-methyladenosine will allow for analysis of methylated transcriptomes, and could aid understanding in how the m6A base modification regulates gene expression.
Application Details
- Application: IP ; DB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4°C
Documentation
Related Tools
References
- • Original hybridoma first published in: Bodi et al. 2010. Nucleic Acids Res. 38(16):5327-35. PMID: 20421205.
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