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Contributor Information

  • Name Wolf Reik
  • Institute Absolute Antibody ; Babraham Institute

Tool Details

  • Tool name: Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] rAb
  • Clone: AB3/63.3
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Reactivity: All
  • Host: Rat
  • Application: IF ; IP ; DB
  • Description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
  • Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
  • Isotype: IgG2a kappa
  • Research area: Genetics

  • For Research Use Only

Target Details

  • Target: 5-Hydroxymethylcytosine (5hmC)
  • Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.

Application Details

  • Application: IF ; IP ; DB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Original hybridoma first published in: Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.