ANTIBODIES

Contributor Information
- Name Ciaran Morrison
- Institute National University of Ireland, Galway ; Centre For Chromosome Biology
Tool Details
- Tool name: Anti-C-NAP1/CEP250 [6F2C8]
- Alternate names: Centrosome-associated protein CEP25; Centrosomal Protein 25; Centrosomal Protein 25kDa; Centrosomal Nek2-Associated Protein 1; 25 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1
- Clone: 6F2C8
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 250
- Application: IHC ; WB ; IHC ; IP ; WB
- Description: Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
- Immunogen: Recombinant human C-NAP1 amino acids 1513-1750 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
- Isotype: IgG1 kappa
- Research area: Cell biology; Immunology
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Centrosomal Nek2-Associated Protein 1
- Target molecular weight: 250
- Target background: Centrosomal Protein 250 (CEP250), also known as Centrosomal Nek2-Associated Protein 1 (C-NAP1), is a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. CEP250 dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
Application Details
- Application: IHC ; WB ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1mg/ml
- Storage buffer: DMEM + 10% FBS + hypoxanthine supplement + non-essential amino acids
- Storage conditions: -20ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Flanagan et al. 2017. Mol Biol Cell. :. PMID: 28100636