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Contributor Information

  • Name Peter Beverley
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-IgM [UCH-B1]
  • Alternate names: B-lymphocytes, IgM, B cell antigen receptor (BCR)
  • Clone: UCH-B1
  • Tool type: Antibodies
  • Tool sub-type: Secondary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 85 kDa
  • Application: IP ; FACS ; IHC ; IF ; Fn
  • Strain: Balb/c
  • Description: Human surface membrane bound IgM complexes with a heterodimer of transmembrane proteins of Ig alpha and Ig beta (CD79a and CD79b) to form the B cell antigen receptor (BCR). The BCR complex plays an important role in B cell development and activation. The transmembrane region of mIgM is essential for functional BCR complex assembly. Antibody UCHB1 recognizes surface expressed and soluble human IgM.
  • Immunogen: Human prolymphocytic leukaemia B cells.
  • Isotype: IgG1
  • Research area: Immunology; Stem cell biology
  • Myeloma used: NS0

  • For Research Use Only

Target Details

  • Target: Prolymphocytic leukaemia B cells Leukemia
  • Target molecular weight: 85 kDa
  • Target background: Human surface membrane bound IgM complexes with a heterodimer of transmembrane proteins of Ig alpha and Ig beta (CD79a and CD79b) to form the B cell antigen receptor (BCR). The BCR complex plays an important role in B cell development and activation. The transmembrane region of mIgM is essential for Fn BCR complex assembly. Antibody UCHB1 recognizes surface expressed and soluble human IgM.

Application Details

  • Application: IP ; FACS ; IHC ; IF ; Fn

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Grupp et al. 1995. J Exp Med. 181(1):161-8. PMID: 7807000.
  •   Molecular mechanisms that control expression of the B lymphocyte antigen receptor complex.
  •   DeFranco et al. 1994. Chem Immunol. 59:156-72. PMID: 7945925.
  •   Structure and function of the B-cell antigen receptor.
  •   Smith-Ravin et al. 1990. Clin Exp Immunol. 82(1):181-7. PMID: 2208792.
  •   Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that discriminate between human mantle zone and marginal zone B cells.
  •   Armitage et al. 1988. Eur J Immunol. 18(1):67-76. PMID: 3257923.
  •   A new antigen identified by the monoclonal antibody UCHB 1 delivers a costimulatory signal to a subset of human B cells.
  •   Coakham et al. 1984. Lancet. 1(8386):1095-8. PMID: 6202990.
  •   Use of monoclonal antibody panel to identify malignant cells in cerebrospinal fluid.