ANTIBODIES

Contributor Information
- Name Peter Beverley
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-IgM [UCH-B1]
- Alternate names: B-lymphocytes, IgM, B cell antigen receptor (BCR)
- Clone: UCH-B1
- Tool type: Antibodies
- Tool sub-type: Secondary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 85 kDa
- Application: IP ; FACS ; IHC ; IF ; Fn
- Strain: Balb/c
- Description: Human surface membrane bound IgM complexes with a heterodimer of transmembrane proteins of Ig alpha and Ig beta (CD79a and CD79b) to form the B cell antigen receptor (BCR). The BCR complex plays an important role in B cell development and activation. The transmembrane region of mIgM is essential for functional BCR complex assembly. Antibody UCHB1 recognizes surface expressed and soluble human IgM.
- Immunogen: Human prolymphocytic leukaemia B cells.
- Isotype: IgG1
- Research area: Immunology; Stem cell biology
- Myeloma used: NS0
- For Research Use Only
Target Details
- Target: Prolymphocytic leukaemia B cells Leukemia
- Target molecular weight: 85 kDa
- Target background: Human surface membrane bound IgM complexes with a heterodimer of transmembrane proteins of Ig alpha and Ig beta (CD79a and CD79b) to form the B cell antigen receptor (BCR). The BCR complex plays an important role in B cell development and activation. The transmembrane region of mIgM is essential for Fn BCR complex assembly. Antibody UCHB1 recognizes surface expressed and soluble human IgM.
Application Details
- Application: IP ; FACS ; IHC ; IF ; Fn
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
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References
- • Grupp et al. 1995. J Exp Med. 181(1):161-8. PMID: 7807000.
- • Molecular mechanisms that control expression of the B lymphocyte antigen receptor complex.
- • DeFranco et al. 1994. Chem Immunol. 59:156-72. PMID: 7945925.
- • Structure and function of the B-cell antigen receptor.
- • Smith-Ravin et al. 1990. Clin Exp Immunol. 82(1):181-7. PMID: 2208792.
- • Characterization of two monoclonal antibodies (UCL4D12 and UCL3D3) that discriminate between human mantle zone and marginal zone B cells.
- • Armitage et al. 1988. Eur J Immunol. 18(1):67-76. PMID: 3257923.
- • A new antigen identified by the monoclonal antibody UCHB 1 delivers a costimulatory signal to a subset of human B cells.
- • Coakham et al. 1984. Lancet. 1(8386):1095-8. PMID: 6202990.
- • Use of monoclonal antibody panel to identify malignant cells in cerebrospinal fluid.