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Contributor Information

  • Name Irene Leigh
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Collagen Type VII [LH7.2]
  • Clone: LH7.2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 250 kDa
  • Application: ELISA ; IHC ; IF ; WB
  • Description: Type VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB.
  • Immunogen: Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions. The epitope is on the NC1 domain.
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Collagen Type VII
  • Target molecular weight: 250 kDa
  • Target background: Type VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB.

Application Details

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Eldardiri et al. 2012. Tissue Eng Part A. 18(5-6):587-97. PMID: 21939396.
  • •  Wound contraction is significantly reduced by the use of microcarriers to deliver keratinocytes and fibroblasts in an in vivo pig model of wound repair and regeneration.
  • •  Al-Refu et al. 2011. Clin Exp Dermatol. 36(1):63-8. PMID: 20637030.
  • •  Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
  • •  Watson et al. 2001. J Invest Dermatol. 116(5):672-8. PMID: 11348454.
  • •  A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
  • •  Shimizu et al. 1990. Br J Dermatol. 122(5):577-85. PMID: 2354110.
  • •  Epidermolysis bullosa acquisita antigen and the carboxy terminus of type VII collagen have a common immunolocalization to anchoring fibrils and lamina densa of basement membrane.