Product Image

Contributor Information

  • Institute A*STAR Accelerate Technologies Pte Ltd

Tool Details

  • Tool name: Anti-NS5B [1A4]
  • Clone: 1A4
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Virus
  • Host: Mouse
  • Application: IF ; WB
  • Description: Hepatitis C virus (HCV) encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV’s RNA dependant RNA polymerase and is critical for viral replication.
  • Immunogen: GST-NS5B (residues 1-346) fusion protein of a HCV genotype 2a strain
  • Isotype: IgG2a
  • Research area: Microbiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Hepatitis C virus non-structural protein 5B
  • Target background: Hepatitis C virus (HCV) encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV’s RNA dependant RNA polymerase and is critical for viral replication.

Application Details

  • Application: IF ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-IgM [Z69]

#153475
Product Image

Anti-MCM5 [A2.2C11.H7.A11.A8]

#151765
Product Image

Anti-TFF1 [PS2GE2]

#151569

References

  • •  Aweya et al. 2015. Virology. 474:41-51. PMID: 25463603.
  • •  NS5B induces up-regulation of the BH3-only protein, BIK, essential for the hepatitis C virus RNA replication and viral release.