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Anti-ORF7a [3C9]

Cat. #152647

Tool name: Anti-ORF7a [3C9]
Clone: 3C9
Tool type: Antibodies
Class: Monoclonal
Conjugate: Unconjugated
Reactivity: Virus
Host: Mouse
Molecular weight of the target: 14 kDa
Application: IF ; WB
Description: Monoclonal antibody used to study the role of ORF7a within SARS coronavirus. Background and Research Application A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals. ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1.
Immunogen: GST-7a (16-111 amino acids)
Immunogen UniProt ID: J9T5H6
Isotype: IgG1
Research area: Cell signaling and signal transduction; Neurobiology
Myeloma used: Sp2/0-Ag14

Target: SARS CoV ORF7a (also known as U122)
Target molecular weight: 14 kDa
Target background: Monoclonal antibody used to study the role of ORF7a within SARS coronavirus. Background and Research Application A novel coronavirus (termed as SARS-CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the essential CoV replication and structural proteins. In addition, the genome encodes eight 8 putative accessory proteins (i.e. ORFs 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b) that do not show significant homology to viral proteins of known coronaviruses, i.e. they are unique to SARS-CoV. It is known to infect humans, bats and various other mammals. ORF7a, also known as U122, consists of 122 amino acids and is localized mainly in ER-Golgi intermediate compartment, where coronaviruses are known to assemble and bud. ORF7a can also be incorporated into the virion. The over-expression of ORF7a induces apoptosis and block cell cycle progression at G0/G1 phase. This is via interfering with pro-survival proteins such as Bcl-XL, Bcl-w, Mcl-1, and A1.

• Tan et al. 2007. J Virol. 81(12):6346-55. PMID: 17428862.
• Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein.

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