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Contributor Information

  • Name Martin Rowe
  • Institute University of Birmingham
  • Primary citation Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.

Tool Details

  • Tool name: Anti-ENTPD1 [AC2]
  • Clone: AC2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 70-100 kDa
  • Application: FACS ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines. ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB-LCL) tested, with a subpopulation of cells in some but not all EB virus genome-positive Burkitt lymphoma lines, but with none of a range of EB virus genome-negative cell lines of lymphoma or leukaemia origin.
  • Immunogen: EBV-transformed human B lymphoblastoid cell line
  • Immunogen UniProt ID: O75356
  • Isotype: IgG1
  • Research area: Tissue-specific biology; Cell biology; Immunology; Neurobiology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)
  • Target molecular weight: 70-100 kDa
  • Target background: ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release.

Application Details

  • Application: FACS ; IF ; IP ; WB
  • Application notes: Recommended Concentrations:
    FACS: 1ug/5x10^5 cells
    Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2ug/ml
    Western blot (ECL): 0.2ug/ml

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.