ANTIBODIES

Contributor Information
- Name Martin Rowe
- Institute University of Birmingham
- Primary citation Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.
Tool Details
- Tool name: Anti-ENTPD1 [AC2]
- Clone: AC2
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 70-100 kDa
- Application: FACS ; IF ; IP ; WB
- Strain: Balb/c
- Description: Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines. ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB-LCL) tested, with a subpopulation of cells in some but not all EB virus genome-positive Burkitt lymphoma lines, but with none of a range of EB virus genome-negative cell lines of lymphoma or leukaemia origin.
- Immunogen: EBV-transformed human B lymphoblastoid cell line
- Immunogen UniProt ID: O75356
- Isotype: IgG1
- Research area: Tissue-specific biology; Cell biology; Immunology; Neurobiology
- Myeloma used: P3X63Ag8.653
- For Research Use Only
Target Details
- Target: Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)
- Target molecular weight: 70-100 kDa
- Target background: ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release.
Application Details
- Application: FACS ; IF ; IP ; WB
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Application notes: Recommended Concentrations:
FACS: 1ug/5x10^5 cells
Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2ug/ml
Western blot (ECL): 0.2ug/ml
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4°C
Related Tools
References
- • Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.