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Contributor Information

  • Institute A*STAR Accelerate Technologies Pte Ltd

Tool Details

  • Tool name: Anti-mib [6B2]
  • Clone: 6B2
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: WB
  • Description: Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells. In addition, there are a series of mib alleles with different genetic severity. The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein.
  • Immunogen: GST fusion-Fish mib protein
  • Isotype: IgG2b
  • Research area: Aquaculture
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: mind bomb
  • Target background: Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells. In addition, there are a series of mib alleles with different genetic severity. The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation