ANTIBODIES

Contributor Information
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti-mib [6B2]
- Clone: 6B2
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Zebrafish
- Host: Mouse
- Application: WB
- Description: Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells. In addition, there are a series of mib alleles with different genetic severity. The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein.
- Immunogen: GST fusion-Fish mib protein
- Isotype: IgG2b
- Research area: Aquaculture
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: mind bomb
- Target background: Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells. In addition, there are a series of mib alleles with different genetic severity. The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein.
Application Details
- Application: WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC