ANTIBODIES
Contributor Information
- Name Ayham Alnabulsi
- Institute Vertebrate Antibodies Limited
Tool Details
- Tool name: Anti-AKT1 [V7]
- Clone: V7
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Zebrafish
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Strain: Balb/c
- Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
- Immunogen: Ovalbumin-conjugated synthetic peptide KDPMQRLGGG (C-terminal sequence)
- Isotype: IgG2a kappa
- Research area: Apoptosis and autophagy; Cancer; Cell biology; Cell signaling and signal transduction; Genetics; Metabolism; Neurobiology
- Myeloma used: P3X63Ag8.653
- For Research Use Only
Target Details
- Target: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
- Target background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
Application Details
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
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