Product Image

Contributor Information

  • Name Dawn Batty ; Rick Wood
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-XPC [RW028]
  • Clone: RW028
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Rabbit
  • Application: IF ; IF ; WB
  • Description: Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.
  • Immunogen: 96-299 of human XPC protein
  • Research area: Genetics

  • For Research Use Only

Target Details

  • Target: Xeroderma Pigmentosum Group C (XPC)
  • Target background: Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.

Application Details

  • Application: IF ; IF ; WB

Handling

  • Format: Liquid
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-FANCC [R7236]

#154178
Product Image

Anti-HPV-18 E6phospho

#153965
Product Image

Anti-KCMF1 (7G2C11G2)

#161944

References

  • •  HCMV-infected cells maintain efficient nucleotide excision repair of the viral genome while abrogating repair of the host genome.
  • •  O'Dowd et al. 2012. PLoS Pathog. 8(11):e1003038. PMID: 23209410.
  • •  O'Dowd et al. 2012. PLoS Pathog. 8(11):e1003038. PMID: 23209410.
  • •  Lange et al. 2009. DNA Repair (Amst). 8(7):865-72. PMID: 19446504.
  • •  Human HMGB1 directly facilitates interactions between nucleotide excision repair proteins on triplex-directed psoralen interstrand crosslinks.
  • •  Louat et al. 2004. FEBS Lett. 574(1-3):121-5. PMID: 15358551.
  • •  Atypical protein kinase C stimulates nucleotide excision repair activity.
  • •  Arajo et al. 2001. Mol Cell Biol. 21(7):2281-91. PMID: 11259578.
  • •  Strong Fn interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome.
  • •  Batty et al. 2000. J Mol Biol. 300(2):275-90. PMID: 10873465.
  • •  Stable binding of human XPC complex to irradiated DNA confers strong discrimination for damaged sites.
  • •  Kberle et al. 1999. Curr Biol. 9(5):273-6. PMID: 10074455.
  • •  Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours.