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Contributor Information

  • Name Ayham Alnabulsi
  • Institute Vertebrate Antibodies Limited

Tool Details

  • Tool name: Anti-RSV Glycoprotein F [11-2-F3]
  • Clone: 11-2-F3
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Virus
  • Host: Mouse
  • Application: ELISA ; IF ; Fn ; WB
  • Strain: Balb/c
  • Description: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa. Immunoblot using the reduced and unreduced RS virus shows that 11-2-F3 reacts with the non-reduced form of the virus F protein (VP70) and the reduced form (F1). It has a plaque reduction neutralization titer of 10 2.2.
  • Immunogen: Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C
  • Isotype: IgG1 kappa, faint lambda
  • Research area: Microbiology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Respiratory Syncytial Virus glycoprotein F
  • Target background: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa. Immunoblot using the reduced and unreduced RS virus shows that 11-2-F3 reacts with the non-reduced form of the virus F protein (VP70) and the reduced form (F1). It has a plaque reduction neutralization titer of 10 2.2.

Application Details

  • Application: ELISA ; IF ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 1mg/ml
  • Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Gimenez et al. 1996. Clin Diagn Lab Immunol. 3(3):280-6. PMID: 8705669. Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224. Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832. Gimenez et al. 1987. J Gen Virol. 68 ( Pt 5):1267-75. PMID: 3572364.