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Contributor Information

  • Name Jonathon Pines
  • Institute University of Cambridge

Tool Details

  • Tool name: Anti-APC15 [APC15]
  • Clone: APC15
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Guinea Pig
  • Molecular weight of the target: 14 kDa
  • Application: IP ; WB
  • Description: The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to S. pombe APC15, and S. cerevisiae Mnd2. hAPC15 is previously uncharacterised. It has been shown for the first time that human APC15 is a component of the Anaphase promoting complex/cyclosome (APC/C) which is required for progression from metaphase during cell cycle. Specifically, APC15 drives the turnover of mitotic checkpoint complexes (MCC)-Cdc20 to make the spindle-assembly checkpoint responsive to kinetochore attachment. Depleting APC15 prevents Cyclin B1 ubiquitylation and degradation because MCCs are locked onto the APC/C and cannot be released when all the kinetochores have attached to the spindle.
  • Immunogen: Full length His-TEVhAPC15 purified from BL21 E.coli
  • Research area: Cancer; Cell biology

  • For Research Use Only

Target Details

  • Target: Human APC15
  • Target molecular weight: 14 kDa
  • Target background: The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to S. pombe APC15, and S. cerevisiae Mnd2. hAPC15 is previously uncharacterised. It has been shown for the first time that human APC15 is a component of the Anaphase promoting complex/cyclosome (APC/C) which is required for progression from metaphase during cell cycle. Specifically, APC15 drives the turnover of mitotic checkpoint complexes (MCC)-Cdc20 to make the spindle-assembly checkpoint responsive to kinetochore attachment. Depleting APC15 prevents Cyclin B1 ubiquitylation and degradation because MCCs are locked onto the APC/C and cannot be released when all the kinetochores have attached to the spindle.

Application Details

  • Application: IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.
  • •  APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.