ANTIBODIES

Contributor Information
- Name Nancy Hogg
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-S100a9 [2B10]
- Clone: 2B10
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mouse
- Host: Rat
- Application: IF ; FACS ; IHC ; IF ; IP ; WB
- Description: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.
- Immunogen: Recombinant full-length murine S100a9
- Isotype: IgG1 kappa
- Research area: Cancer; Cell signaling and signal transduction; Immunology
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: S100a9
- Target background: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.
Application Details
- Application: IF ; FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Colom et al. 2015. Immunity. 42(6):1075-86. PMID: 26047922.
- • Leukotriene B4-Neutrophil Elastase Axis Drives Neutrophil Reverse Transendothelial Cell Migration In Vivo.
- • Chen et al. 2015. Mucosal Immunol. 8(2):390-402. PMID: 25160819.
- • IL-23 activates innate lymphoid cells to promote neonatal intestinal pathology.
- • Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
- • Voisin et al. 2010. Am J Pathol. 176(1):482-95. PMID: 20008148.
- • Venular basement membranes ubiquitously express matrix protein low-expression regions: characterization in multiple tissues and remodeling during inflammation.
- • Young et al. 2007. Br J Pharmacol. 151(5):628-37. PMID: 17471175.
- • Role of neutrophil elastase in LTB4-induced neutrophil transmigration in vivo assessed with a specific inhibitor and neutrophil elastase deficient mice.
- • Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
- • Myeloid cell function in MRP-14 (S100A9) null mice.