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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-S100a9 [2B10]
  • Clone: 2B10
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse
  • Host: Rat
  • Application: IF ; FACS ; IHC ; IF ; IP ; WB
  • Description: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.
  • Immunogen: Recombinant full-length murine S100a9
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell signaling and signal transduction; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: S100a9
  • Target background: The mAb is specific for mouse S100a9 protein that forms a heterodimer with S100a8 and constitutes 45% of the neutrophil cytosolic protein. The mAb stains neutrophils strongly and monocytes weakly.

Application Details

  • Application: IF ; FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Colom et al. 2015. Immunity. 42(6):1075-86. PMID: 26047922.
  •   Leukotriene B4-Neutrophil Elastase Axis Drives Neutrophil Reverse Transendothelial Cell Migration In Vivo.
  •   Chen et al. 2015. Mucosal Immunol. 8(2):390-402. PMID: 25160819.
  •   IL-23 activates innate lymphoid cells to promote neonatal intestinal pathology.
  •   Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
  •   Voisin et al. 2010. Am J Pathol. 176(1):482-95. PMID: 20008148.
  •   Venular basement membranes ubiquitously express matrix protein low-expression regions: characterization in multiple tissues and remodeling during inflammation.
  •   Young et al. 2007. Br J Pharmacol. 151(5):628-37. PMID: 17471175.
  •   Role of neutrophil elastase in LTB4-induced neutrophil transmigration in vivo assessed with a specific inhibitor and neutrophil elastase deficient mice.
  •   Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
  •   Myeloid cell function in MRP-14 (S100A9) null mice.