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Contributor Information

  • Name Caetano Reis e Sousa
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CLEC2 [17D9]
  • Clone: 17D9
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse
  • Host: Rat
  • Application: FACS ; WB
  • Description: Monoclonal antibody used to study the expression pattern of CLEC-2 receptor.
  • Immunogen: RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2
  • Immunogen UniProt ID: Q9P126
  • Isotype: IgG2b
  • Research area: Tissue-specific biology; Immunology
  • Myeloma used: Y3.AG.1.2.3

  • For Research Use Only

Target Details

  • Target: Ctype lectin-like receptor (CLEC2)
  • Target background: CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower level on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. CLEC-2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC-2 also functions as an activation receptor on monocytes and neutrophils to induce phagocytosis and proinflammatory cytokine production. This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.

Application Details

  • Application: FACS ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Kerrigan et al. 2009. J Immunol. 182(7):4150-7. PMID: 19299712.
  •   CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.