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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Nanog [Nanog]
  • Clone: Nanog
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Rabbit
  • Application: ChIP ; FACS ; IF ; IP ; WB
  • Description: Nanog is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. Human NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. Overexpression of Nanog in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. In the absence of Nanog, mouse embryonic stem cells differentiate into visceral/parietal endoderm. Loss of Nanog function causes differentiation of mouse embryonic stem cells into other cell types. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
  • Immunogen: Purified GST-Nanog 1-95 fusion protein
  • Research area: Cancer; Cell biology; Developmental biology; Genetics; Stem cell biology

  • For Research Use Only

Target Details

  • Target: Nanog
  • Target background: Nanog is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. Human NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. Overexpression of Nanog in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. In the absence of Nanog, mouse embryonic stem cells differentiate into visceral/parietal endoderm. Loss of Nanog function causes differentiation of mouse embryonic stem cells into other cell types. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.

Application Details

  • Application: ChIP ; FACS ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1.5 mg/ml
  • Storage buffer: Whole serum
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Torres et al. 2008. Nat Cell Biol. 10(2):194-201. PMID: 18223644.
  • •  Nanog maintains pluripotency of mouse embryonic stem cells by inhibiting NFkappaB and cooperating with Stat3.