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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Goblet cells [FIS 3G12/3]
  • Clone: FIS 3G12/3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Molecular weight of the target: 175 kDa, 120 kDa, 70 kDa
  • Application: IHC ; IF ; WB
  • Strain: Balb/c
  • Description: 3G12 is a marker for mucus-secreting cells in the posterior gut of zebrafish.
  • Immunogen: Lysate of fish gut intestine
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Goblet cells in the posterior part of the zebrafish intestine
  • Target molecular weight: 175 kDa, 120 kDa, 70 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 3G12 marks goblet cells specifically. It labels both the mucus compartment and other parts of the cytoplasm. On Western blots, 3G12 stains diffuse bands, consistent with variably glycosylated antigen(s). 3G12 labelled cells are concentrated in the posterior gut.

Application Details

  • Application: IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  •   Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.