ANTIBODIES
Contributor Information
- Name Jacqueline Cordell
- Institute University of Oxford
Tool Details
- Tool name: Anti-CD31 (PECAM1) [JC70] mAb
- Alternate names: Platelet/Endothelial Cell Adhesion Molecule 1; CD31 Antigen; EndoCAM; PECAM-1; GPIIA; PECA1; CD31/EndoCAM; CD31
- Clone: JC70
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IHC ; IF ; WB
- Description: Monoclonal antibody directed against endothelial cell marker (CD31, JC70), capable of evaluating tumour angiogenesis and angiosarcoma more effectively than anti-FVIII-Reg.
- Immunogen: Cell membrane preparation from the spleen of a patient with hairy cell leukaemia
- Immunogen UniProt ID: P16284
- Isotype: IgG1
- Research area: Cell biology; Cancer; Tissue-specific biology; Cell signaling and signal transduction; Stem cell biology
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: PECAM1 (CD31)
- Target background: CD31/JC70, also known as platelet endothelium cell adhesion molecule (PECAM-1), is a 130kDa transmembrane glycoprotein found on cell surface of endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. PECAM1 is a member of the immunoglobulin (Ig) superfamily. It is also a major constituent of the endothelial cell intercellular junction and expressed in certain cancers. PECAM1 is involved in cell signalling and adhesion, trans-endothelial migration of leukocytes, angiogenesis, and integrin activation. PECAM1 is a specific marker of endothelial differentiation. Anti-CD31 be used to identify the presence of endothelial cells in a histological tissue sample to determine the degree of angiogenesis (which in turn can determine tumour severity/growth). Anti-CD31 stains a fixation resistant epitope on endothelial cells in both benign and malignant endothelial cells in angiosarcomas with more consistency than monoclonal or polyclonal antibodies to FV111-Rag . CD31 is a good marker of endothelial cells in paraffin sections. Anti-CD31 was created to recognise membrane bound glycoprotein CD31/JC70.
Application Details
- Application: FACS ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Documentation
Related Tools
References
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- • JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
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