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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Zebrafish basement membrane marker [FIS 5F11/2]
  • Clone: FIS 5F11/2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: 5F11 is a marker for the basement membrane of the intestinal epithelium.
  • Immunogen: Zebrafish Intestine
  • Isotype: IgG1
  • Research area: Cell biology; Developmental biology; Tags and cell markers
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Zebrafish basement membrane marker
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  •   Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.