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Contributor Information

  • Name Michael Tilby
  • Institute The Institute of Cancer Research; Newcastle University

Tool Details

  • Tool name: Anti-Melphalan-modified DNA [MP5/73]
  • Clone: MP5/73
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Rat
  • Application: ELISA ; IHC
  • Description: This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
  • Immunogen: Native DNA reacted with melphalan
  • Isotype: IgG2b
  • Research area: Cancer; Genetics
  • Myeloma used: Y3.AG.1.2.3

  • For Research Use Only

Target Details

  • Target: Melphalan
  • Target background: This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.

Application Details

  • Application: ELISA ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Gould et al. 2004. Mol Pharmacol. 66(5):1301-9. PMID: 15308759.
  • •  p53 elevation in relation to levels and cytotoxicity of mono- and biFn melphalan-DNA adducts.
  • •  Rothbarth et al. 2004. Biochem Pharmacol. 67(9):1771-8. PMID: 15081876.
  • •  Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo.
  • •  Frank et al. 2003. Exp Cell Res. 283(2):127-34. PMID: 12581733.
  • •  Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation.
  • •  McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.
  • •  Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.
  • •  Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.
  • •  A monoFn derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.
  • •  Frank et al. 1996. Blood. 88(3):977-84. PMID: 8704257.
  • •  Detection and quantification of melphalan-DNA adducts at the single cell level in hematopoietic tumor cells.
  • •  Tilby et al. 1993. Eur J Cancer. 29A(5):681-6. PMID: 8471325.
  • •  Application of a sensitive immunoassay to the study of DNA adducts formed in peripheral blood mononuclear cells of patients undergoing high-dose melphalan therapy.
  • •  Tilby et al. 1990. Chem Biol Interact. 73(2-3):183-94. PMID: 2155711.
  • •  Alkylation of DNA by melphalan in relation to immunoassay of melphalan-DNA adducts: characterization of mono-alkylated and cross-linked products from reaction of melphalan with dGMP and GMP.
  • •  Tilby et al. 1987. Cancer Res. 47(6):1542-6. PMID: 3815354.
  • •  Immunological detection of DNA damage caused by melphalan using monoclonal antibodies.