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Contributor Information

  • Name Margaret Goodall ; D.L. Hardie ; Roy Jefferis ; Ian MacLennen
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-Rough ER Component [BU18]
  • Clone: BU18
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Strain: Balb/c
  • Description: All eukaryotic cells have an endoplasmic reticulum (ER). Its membrane typically constitutes more than half of the total membrane of an average animal cell. The ER is organized into a netlike labyrinth of branching tubules and flattened sacs extending throughout the cytosol. The tubules and sacs are all thought to interconnect, so that the ER membrane forms a continuous sheet enclosing a single internal space. This highly convoluted space is called the ER lumen or the ER cisternal space, and it often occupies more than 10% of the total cell volume. The ER membrane separates the ER lumen from the cytosol, and it mediates the selective transfer of molecules between these two compartments.
  • Isotype: IgG1
  • Research area: Cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Rough endoplasmic reticulum component
  • Target background: All eukaryotic cells have an endoplasmic reticulum (ER). Its membrane typically constitutes more than half of the total membrane of an average animal cell. The ER is organized into a netlike labyrinth of branching tubules and flattened sacs extending throughout the cytosol. The tubules and sacs are all thought to interconnect, so that the ER membrane forms a continuous sheet enclosing a single internal space. This highly convoluted space is called the ER lumen or the ER cisternal space, and it often occupies more than 10% of the total cell volume. The ER membrane separates the ER lumen from the cytosol, and it mediates the selective transfer of molecules between these two compartments.

Application Details

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: RPMI 1640 + 10% FCS
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation