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Contributor Information

  • Name Linda Ariza-McNaughton
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]
  • Clone: FIS 2F11/2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Zebrafish
  • Host: Mouse
  • Molecular weight of the target: 170 kDa, 120 kDa, 90 kDa and 35 kDa
  • Application: IHC ; ELISA ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium.
  • Immunogen: Lysate of zebrafish intestine
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Tags and cell markers; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Zebrafish gut secretory cell epitopes
  • Target molecular weight: 170 kDa, 120 kDa, 90 kDa and 35 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels all the gut secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants. The antibody also labels hepatopancreatic ducts, intrahepatic bile ducts and enteroendocrine. Anti-2F11 provides an early marker to distinguish cell types and cell polarity in the zebrafish gut.

Application Details

  • Application: IHC ; ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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