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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CD11 & CD18 [24]
  • Alternate names: Integrin Subunit Alpha L; Leukocyte Function-Associated Molecule 1 Alpha Chain; CD11 Antigen-Like Family Member A; Antigen CD11A (P18); LFA-1A; CD11A; Antigen CD11A (P18); Lymphocyte Function-Associated Antigen 1; Lymphocyte Function-Associated Antigen 1; Alpha Polypeptide; Integrin Gene Promoter; Alpha Polypeptide; Integrin Alpha L; CD11a Antigen; LFA-1; LFA1A
  • Clone: 24
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 95 kDa and 180 kDa
  • Application: FACS ; IHC ; IF ; IP ; RIA ; WB
  • Strain: Balb/c
  • Description: Clone 24 may be used as a reporter of the activation state of the CD11/CD18 integrins and can also be induced following exposure with Mg2+ or Mn2+.
  • Immunogen: Fibronectin-purified human monocytes .
  • Isotype: IgG1
  • Research area: Cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Activation epitope on integrin beta 2(CD18) subunit
  • Target molecular weight: 95 kDa and 180 kDa
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Integrin beta2 (CD18) associates with integrin alpha L (CD11a) to form the leukocyte function-associated antigen-1 (LFA-1), integrin alpha M (CD11b) to form Complement Receptor 3 (CR3), integrin alpha X (CD11c) to form Complement Receptor 4 (CR4) and with CD11d to form p150,95. Each integrin receptor can bind various Intercellular adhesion molecules (ICAMs) and matrix molecules.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Drewry et al. 2019. Nat Microbiol. 4(11):1951-1963. PMID: 31332383.
  • •  Chen et al. 2019. Nanoscale. 11(12):5377-5394. PMID: 30849160.
  • •  Yuan et al. 2018. Eur J Cardiothorac Surg. 53(2):400-408. PMID: 28950359.
  • •  Laufer et al. 2018. Front Immunol. 9:3115. PMID: 30692994.
  • •  Manwani et al. 2015. Am J Hematol. 90(5):381-5. PMID: 25616042.
  • •  Chen et al. 2010. Proc Natl Acad Sci U S A. 107(33):14727-32. PMID: 20679211.
  • •  Requirement of open headpiece conformation for activation of leukocyte integrin alphaXbeta2.
  • •  Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
  • •  Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
  • •  Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
  • •  A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
  • •  McDowall et al. 2003. J Clin Invest. 111(1):51-60. PMID: 12511588.
  • •  A novel form of integrin dysfunction involving beta1, beta2, and beta3 integrins.
  • •  Hogg et al. 1985. Cell Immunol. 92(2):247-53. PMID: 2581704.
  • •  An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue.