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Contributor Information

  • Name Jacqueline Cordell
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-CD79a [HM47] mAb
  • Alternate names: B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
  • Clone: HM47
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Bovine ; Chicken ; Horse ; Human ; Guinea Pig ; Mouse ; Opossum ; Pig ; Primate ; Rat ; Rabbit
  • Host: Mouse
  • Application: IHC ; IP ; WB
  • Description: The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.
  • Immunogen: Synthetic peptide of 14 amino acids representing amino acids 202-216 of the human mb-1 cDNA sequence
  • Isotype: IgG1
  • Research area: Cell biology; Cancer; Cell signaling and signal transduction; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: CD79a
  • Target background: The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.

Application Details

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

Documentation

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References

  • •  Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
  • •  Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
  • •  Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
  • •  The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
  • •  Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
  • •  The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
  • •  van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
  • •  The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.