Product Image

Contributor Information

  • Name Michael Tilby
  • Institute The Institute of Cancer Research; Newcastle University

Tool Details

  • Tool name: Anti-Melphalan-modified DNA [Amp4/42]
  • Clone: Amp4/42
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Rat
  • Application: ELISA ; IF
  • Description: Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali
  • Isotype: IgG2b
  • Research area: Cancer; Genetics
  • Myeloma used: Y3.AG.1.2.3

  • For Research Use Only

Target Details

  • Target: Melphalan
  • Target background: The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.

Application Details

  • Application: ELISA ; IF

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-ENTPD1 [AC2]

#151580
Product Image

Anti-DNA [m17-s-c3]

#157795
Product Image

Anti-Blood Group A [mbbm1]

#151693

References

  • •  Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15
  • •  283(2):127-34.PMID: 12581733
  • •  Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.
  • •  Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.
  • •  McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.
  • •  McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.
  • •  Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.
  • •  Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.
  • •  A monoFn derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.
  • •  Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.
  • •  A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.