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Contributor Information

  • Name Nick Tidman
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Keratin6 [LHK6]
  • Alternate names: CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E Human Entrez Gene ID 3853-KRT6A, 3854-KRT6B, 286887-KRT6C Human SwissProt P2538-KRT6A, P4259-KRT6B, P48668-KRT6C Human Unigene 7779 Human Gene Symbol KRT6A; KRT6B; KRT6C Human Chromosome Location 12q13.13; 12q13.13; 12q13.13
  • Clone: LHK6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Molecular weight of the target: 55-57 kDa
  • Application: ELISA ; FACS ; IHC ; IF ; WB
  • Description: LHK6 is a very good marker for hyperproliferative keratinocytes.
  • Immunogen: 11 amino aid sequence GSSTIKYTTTS of K6b C terminal
  • Isotype: IgG2a kappa
  • Research area: Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Keratin 6
  • Target molecular weight: 55-57 kDa
  • Target background: Clone LHK6 recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratin 9 to 23) and on type II kerating (1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 6,16 and 17 are expressed in suprabasasl keratinocytes of wounded epidermis. Keratin 6 is found in acrosyringeal keratinocyes in normal skin.

Application Details

  • Application: ELISA ; FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Toivola et al. 2002. Mol Biol Cell. 13(6):1857-70. PMID: 12058054.
  • •  Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells.
  • •  Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
  • •  K15 expression implies lateral differentiation within stratified epithelial basal cells.
  • •  Machesney et al. 1998. Am J Pathol. 152(5):1133-41. PMID: 9588880.
  • •  Activated keratinocytes in the epidermis of hypertrophic scars.
  • •  Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
  • •  Basal cell-specific and hyperproliferation-related keratins in human breast cancer.