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Contributor Information

  • Name Roy Jefferis
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-Integrin aX [BU15]
  • Clone: BU15
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Dog ; Human ; Primate
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP
  • Strain: Balb/c
  • Description: Monoclonal antibody directed against integrin aX, with capability in diagnosing hairy cell leukaemia.
  • Immunogen: Dendritic cells of synovial fluid
  • Immunogen UniProt ID: P20702
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Integrin alpha X (CD11C)
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha X is found on hairy cell leukaemias, malignant histiocytes, on tissue macrophages, monocytes, activated neutrophils, monocyte derivative dendritic cells and some T-cell clones. Abnormal expression of Integrin aX is characteristic of hairy cell leukaemia (HCL) and requires activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin aX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. BU15 has utility for the diagnosis of hairy cell leukaemia.

Application Details

  • Application: FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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