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Contributor Information

  • Name Jacqueline Cordell
  • Institute University of Oxford
  • Primary citation Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 197911

Tool Details

  • Tool name: Anti-BCL2 [bcl-2/100] mAb
  • Clone: bcl-2/100
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; WB
  • Description: Monoclonal antibody directed against both isoforms of BCL-2 protein.
  • Immunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
  • Immunogen UniProt ID: P10415
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell signaling and signal transduction; Metabolism
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Bcl2
  • Target background: The pro-survival protein BCL2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. There are two isoforms, alpha and beta, of Bcl-2 have been identified and are a result of alternative splicing at the C-terminus. BCL-2 protein is involved in apoptosis, and inhibits the function of pro-apoptotic proteins such as Bax and Bak. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukaemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments. This antibody recognises both the alpha and beta isoforms of BCL-2.

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 8 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Hirpara et al. 2020. Redox Biol. 30:101403. PMID: 31954371.
  • •  Steinbach et al. 2020. PLoS One. 15(1):e0227676. PMID: 31935257.
  • •  Jeffery et al. 2017. Clin Exp Immunol. 188(3):394-411. PMID: 28176332.
  • •  Soilu-Hänninen et al. 1999. J Neurosci. 19(12):4828-38. PMID: 10366617.
  • •  Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.
  • •  Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.
  • •  Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.
  • •  Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.
  • •  de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.
  • •  Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.
  • •  Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.
  • •  Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.
  • •  Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.
  • •  Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.
  • •  Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.